2021年5月11日，国家香蕉产业技术体系品种改良岗位的论文“Elucidating the mechanism of MaGWD1-mediated starch degradation cooperatively regulated by MaMADS36 and MaMADS55 in banana”被国际Top学术期刊《Postharvest Biology and Technology》(SCI，JCR一区，IF=4.303) 录用发表。岗位专家金志强研究员和刘菊华研究员为通讯作者。该项工作得到了国家香蕉产业技术体系专项资金 (CARS-31) 支持。

　　作者：刘菊华*，刘梦婷，贾彩红，张静，苗红霞，王静毅，张建斌，王卓，徐碧玉，李新国*，金志强*

　　题目：MaMADS36和MaMADS55协同调控MaGWD1基因介导香蕉果实淀粉降解的分子机制

　　摘要：香蕉 (Musa acuminata) 是一种常见的淀粉转化型水果，其淀粉转化效率直接影响采后果实硬度、甜度和货架期等。葡聚糖水合二激酶 (glucan water dikinase，GWD) 是淀粉降解代谢的第一个限速酶，但其在香蕉果实采后淀粉降解中的作用及分子机制尚不清楚。本研究解析了MaMADS36和MaMADS55协同调控MaGWD1表达介导香蕉采后果实淀粉降解的分子机制。结果表明：MaMADS36与MaMADS55相互作用，可以形成复合体；MaGWD1在香蕉果实采后淀粉降解过程中扮演重要的角色；MaMADS36和MaMADS55均可以直接与MaGWD1启动子的CA/T(r) G结合；MaMADS36和MaMADS55以复合体形式协同调控MaGWD1的表达，进而促进香蕉果实淀粉降解。这些发现为我们了解TFs如何调控果实淀粉降解提供了理论依据，丰富了香蕉果实淀粉降解代谢的调控网络；同时，也为香蕉果实品质改良，促进香蕉产业发展提供了重要的基因资源。

　　全文链接：https://doi.org/10.1016/j.postharvbio.2021.111587

　　Juhua Liu*, Mengting Liu, Caihong Jia, Jing Zhang, Hongxia Miao, Jingyi Wang, Jianbin Zhang, Zhuo Wang, Biyu Xu, Xinguo Li*, Zhiqiang Jin*. Elucidating the mechanism of MaGWD1-mediated starch degradation cooperatively regulated by MaMADS36 and MaMADS55 in banana. Postharvest Biology and Technology, 2021, 179: 111587.

　　Abstract

　　Banana (Musa acuminata) is a common starch-conversion fruit. Starch is rapidly degraded and converted to soluble sugars during postharvest banana fruit ripening. The conversion efficiency directly influences fruit firmness, sweetness, and shelf life. Glucan, water dikinase (GWD) is the first rate-limiting enzyme of starch metabolism. Musa acuminata glucan water dikinase 1 (MaGWD1) plays an important role in banana starch metabolism and its expression is regulated by transcription factors (TFs). However, the regulation of MaGWD1 by MADS-box TFs remain unclear. In this study, the molecular mechanism underlying the cooperative regulation of MaGWD1-mediated starch degradation by MaMADS36 and MaMADS55 was investigated. The results indicated that MaMADS36 interacts with MaMADS55. Both MaMADS36 and MaMADS55 could directly bind to the same position on the CA/T(r)G box within the MaGWD1 promoter. MaMADS36 and MaMADS55 cooperatively regulate banana fruit starch degradation via transcriptional control of MaGWD1. These findings improve our understanding of how TFs regulate starch degradation; this information may be used for banana fruit quality improvement and to support the development of the banana industry.

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